Osaka scientists develop wild yeast that boosts ornithine in craft beer
Researchers in Japan say they have developed a naturally derived yeast strain capable of producing significantly higher levels of ornithine, an amino acid commonly used in health supplements, in a bid to tap into the trend for value-added fermented drinks.
The team, based in Osaka, says the new strain could allow craft brewers to produce beers with enhanced levels of the compound without using genetic modification, phys.org has reported.
Their findings were published in the Journal of Industrial Microbiology and Biotechnology on 20 May 2026.
Ornithine plays a role in the body’s urea cycle, helping to remove ammonia, a waste product produced during protein metabolism. It is also marketed in some health supplements that claim to reduce fatigue, ease stress and improve sleep quality, although the effectiveness of such products can vary.
The researchers, led by Akira Nishimura, Shota Isogai, Koya Yamada, Ryoya Tanahashi and Hiroshi Takagi, developed the new yeast strain, known as ADHorn49, using conventional breeding techniques rather than gene editing.
The strain was derived from ADH837, a naturally occurring Saccharomyces cerevisiae yeast originally isolated through a collaboration between the Nara Institute of Science and Technology (NAIST) and Osaka-based company 10 Fields Factory.
Instead of modifying the yeast’s DNA directly, the team used chemical mutagenesis to generate naturally occurring mutations before selecting promising candidates. Laboratory testing showed that ADHorn49 accumulated more than nine times as much ornithine inside its cells as its parent strain.
According to the researchers, the mutation appears to alter the yeast’s normal metabolic regulation without affecting its suitability for brewing.
Trials using brewing wort found the strain produced similar levels of carbon dioxide to the original yeast while releasing substantially more ornithine during fermentation, reaching 7.0mg per litre after four days.
Value-added beers
The yeast has already been tested in commercial-style brewing.
Using ADHorn49, 10 Fields Factory produced a prototype golden strong ale containing 19.0mg of ornithine per litre, compared with 6.1mg per litre in beer brewed with the standard NAIST yeast strain.
According to the scientists, the work could provide craft brewers with a way to create value-added beers using conventional breeding methods, potentially appealing to consumers seeking products made without genetic modification.
“This study clearly demonstrates a practical non-genetically modified strategy that combines traditional microbial breeding with molecular understanding,” professor Hiroshi Takagi told phys.org. “By linking natural-environment yeast resources with modern fermentation biotechnology, we hope to support the development of value-added fermented foods and beverages.”
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